On the night of 27/28 February 1991, within hours of the ceasefire, two General Dynamics F-111Fs, loaded with one GBU-28 each, headed towards a target on the outskirts of Baghdad. The al-Taji Airbase, located 15 mi (27.4 km) northwest of the Iraqi capital, had been hit at least three times by GBU-27/Bs from F-117 Nighthawks, "digging up the rose garden".[9] The first GBU-28 was dropped off-target due to target misidentification. The second GBU-28 was a direct hit and penetrated the thick reinforced concrete before detonating, killing everyone inside.
The bomb was used during Operations Enduring Freedom in 2002 and Iraqi Freedom in 2003 by USAF F-15Es.